Accession Number:

ADA238677

Title:

Structural Role of Amino Acids 99-110 in Recombinant Human Erythropoietin

Descriptive Note:

Corporate Author:

NEW ENGLAND DEACONESS HOSPITAL BOSTON MA

Report Date:

1990-01-01

Pagination or Media Count:

30.0

Abstract:

Erythropoietin is the prime regulator of red blood cell production. Mutagenesis of wild type human erythropoietin cDNA was used to produce a series of mutant proteins with sequential deletion of three adjacent amino acids and insertion of the sequence Glu-Phe across the two domains. Transient expression in COS-7 cells revealed 2.0 kb transcripts encoded by all of the cDNas.

Subject Categories:

  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE