Accession Number:

ADA238603

Title:

Development of Hematopoietic Growth Factors for Use in Military Personnel

Descriptive Note:

Status rept. 15 Apr-31 May 90,

Corporate Author:

NEW ENGLAND DEACONESS HOSPITAL BOSTON MA

Personal Author(s):

Report Date:

1990-08-17

Pagination or Media Count:

2.0

Abstract:

Mutation of amino acids 99-109 of the hormone drastically alters the hormones biological activity. Our preliminary conclusion is that amino acids 99-109 are very important for biological activity. The goal of this project is to purify the B cell derived burst promoting activity B-BPA growth factor in order to obtain amino acid sequence, clone and express the gene, and initiate in vivo trials of this agent. We have begun to develop an animal model for the human B-BPA by testing conditioned medium from lymphocyte cultures of mice. At present we are developing the in vitro assays of mouse erythroid progenitor cells necessary to measure murine B-BPA. As soon as we are confident in our ability to carry out these assays, we will be able to begin to identify and characterize murine and other animal B-BPAs and initiate a purification program.

Subject Categories:

  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE