Accession Number:

ADA238553

Title:

Mutant Proteins--Enzymes to Hydrolyze Toxic Organophosphates

Descriptive Note:

Final rept. 15 Jun 1983-14 Jun 1989

Corporate Author:

CALIFORNIA INST OF TECH PASADENA DIV OF CHEMISTRY AND CHEMICAL ENGINEERING

Personal Author(s):

Report Date:

1991-06-17

Pagination or Media Count:

30.0

Abstract:

This work focuses on the creation of new catalytic activities by modification of existing enzymes. To this end, techniques for the synthesis of large genes have been developed together with a complementation system for the expression and correct folding of proteins that are normally self-processing but that have mutated so they can no longer process themselves. Concurrent expression of the pro-sequence and the mature protein sequence encoded on separate but compatible plasmids accomplishes this purpose the pro-sequence apparently performs the role of a chaperoning in this case. The enzymes studied are two serine hydrolases, alpha protease and B-lactamase. For a-lytic proteases the new activity sought was the ability to hydrolyze organophosphates rather than being irreversibly inhibited by these substances. For Beta lactamase we created a structural variant that had a dramatically different catalytic role on the normal beta lactam substrates.

Subject Categories:

  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE