World Reference Center for Arboviruses and Retroviruses
Final rept. 9 Feb 1987-8 Feb 1990
YALE UNIV NEW HAVEN CT SCHOOL OF MEDICINE
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The World Reference Center for Arboviruses and Retroviruses received viruses from the United States and foreign countries for characterization and identification. New viruses, or viruses causing diseases not previously recognized included a subtype of Cache Valley virus from a febrile military recruit, LaCrosse virus from encephalitic dogs, Kagoshima and Cache Valley viruses associated with fetal abnormalities of livestock, and new phleboviruses from tropical America and West Africa. Genetically engineered flavivirus trpE proteins reacted specifically by ELISA. The cell lysate antigen technique for ELISA was adapted to a large number of arboviruses. ELISA was also developed for rapid testing of 17D yellow fever vaccinees. Limited primer extension sequencing of dengue and Japanese encephalitis viruses showed that their genotypes were geographically focal. The method was used to demonstrate the origin of an introduced dengue virus.
- Medicine and Medical Research