Development of Hematopoietic Growth Factors for Use in Military Personnel
Rept. for 1 Jun-30 Sep 1990
HARVARD MEDICAL SCHOOL BOSTON MA
Pagination or Media Count:
This project is designed to develop second generation erythropoietin molecules with more favorable pharmacologic properties that can be applied to military personnel. Part of our strategy has been to study the structure of the molecule using a combination of chemical and molecular biologic techniques in order to rationally engineer new bio-molecules. When this grant began, we had evidence that amino acids 99-109 of the hormone were important for its biological activity. In the last four months, we have completed our initial series of experiments and have found that the amino acids in this domain are important both for structural stability and receptor recognition. Mutation of these amino acids to others which significantly alter the structure of this area of the molecule result in recombinant erythropoietin that are more susceptible to proteolytic cleavage inside the secreting cell.
- Medicine and Medical Research