Image Analysis of Viral-Expressing Mouse Macrophage Cells
Final rept. 1 Jul 90-31 Mar 91,
TRINITY UNIV SAN ANTONIO TX DEPT OF BIOLOGY
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RAW 264.7 mouse macrophage cells have interesting growth properties. To best utilize these cells in research protocols, rapid and inexpensive monitoring of in situ growth characteristics are necessary. An inexpensive computer imaging and visualization system was assembled to complement the microscopical analysis if in situ cells. Data for image analysis was provided by a combination of optically scanned cell cultures, framed-grabbed light microscopy, and optically scanned scanning and transmission micrographs of in situ cell cultures. The imaging system proved to be quite rapid. Several elements concerning RAW cell growth were uncovered including a relationship between cell culture seed concentration and the appearance of foci. However, the mechanism of viral expression is still unclear. The imaging system was successfully adapted for looking at bacterial cultures as well. A low-cost imaging system is now in place for examining in situ cell culture systems of several types.
- Medicine and Medical Research