Development of Biochemical Cyanide Antidotes
Midterm rept. 1 Feb 1989-1 Nov 1990
PURDUE RESEARCH FOUNDATION LAFAYETTE IN
Pagination or Media Count:
Twenty-four different substances were tested as potential cyanide antidotes using cultured rat pheochromocytoma p-PC12 cells. Prevention of cyanide-induced biochemical changes increased cell calcium, peroxide formation, cytochrome oxidase inhibition and inhibition of antioxidant defense enzymes in isolated PC12 cells was used as a measure of in vitro antidotal effectiveness. For comparison, protection against cyanide lethality in mice LD50 was evaluated for -11 compounds. The linear regression line relating in vitro with in vivo antidotal activity had a correlation coefficient of 0.54. Thus more data are needed to fully evaluate the in vitro system but this rapid screening procedure may prove to be an efficient way to predict whether a substance is a useful cyanide antagonist.
- Chemical, Biological and Radiological Warfare