Surface-Bound Biomembranes Incorporating Receptors: Electrochemical and Structural Characterization.
TSI MASON RESEARCH INST WORCESTER MA BIOCHEMISTRY DEPT
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A method is described for forming stable surface-bound structures that incorporate membrane receptors in a lipid bilayer environment. These structures were formed by dialysis of detergent-solubilized biomembranes in the presence of two alkylsilanated substrates SiSiO2 and glass beads. Electrochemical analysis of the capacitance was used to determine an apparent thickness and the degree of surface coverage at each stage in the deposition process. Similarly, elemental analysis on glass beads was used to quantitate the carbon load. Glass beads substrates were also examined by FTIR spectroscopy to evaluate the alkylsilanated substrate before and after dialysis.
- Medicine and Medical Research