Accession Number:

ADA237527

Title:

Analysis of Nearest Neighbor Interactions in the Pyrimidine Triple Helix Motif by Affinity Cleaving

Descriptive Note:

Technical rept. no. 4, 31 May 90-1 Jun 91,

Corporate Author:

CALIFORNIA INST OF TECH PASADENA DIV OF CHEMISTRY AND CHEMICAL ENGINEERING

Personal Author(s):

Report Date:

1991-06-01

Pagination or Media Count:

13.0

Abstract:

Oligonucleotide-directed sequence specific recognition of double helical DNA is length and sequence composition dependent. In order to dissect the relative importance of nearest neighbor interactions on triplet stabilities, binding by oligonucleotides of different sequence composition has been characterized by affinity cleaving. Several groups of target duplexes were synthesized with a central base triplet held constant and the adjacent 3 and 5 triplets systematically varied. By incorporating a thymidine residue with the Deovy Ribonucleic acids DNA-cleaving moiety EDTA in the third strand, the relative stabilities of Hoogsteen base triplets T.AT and CGC as well as the newly discovered base triplets G.TA, Z.TA, and D3.CG were assessed in the context of different sequences.

Subject Categories:

  • Biochemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE