Accession Number:

ADA237360

Title:

Recognition of All Four Base Pairs of Duplex DNA by Triple Helix Formation. Design of Pyrimidine Specific Bases

Descriptive Note:

Technical rept. 31 May 90-1 Jun 91,

Corporate Author:

CALIFORNIA INST OF TECH PASADENA DIV OF CHEMISTRY AND CHEMICAL ENGINEERING

Report Date:

1991-06-01

Pagination or Media Count:

22.0

Abstract:

The novel base 4-3-benzamidophenylimidazole was designed, synthesized and incorporated within a pyrimidine oligonucleotide and shown to recognize pyrimidine purine base pairs over purine pyrimidine base pairs. Such specificity allows binding by triple helix formation at an 18 base pairs site in simian virus 40 DNA containing all four base pairs at physiologically relevant conditions.

Subject Categories:

  • Genetic Engineering and Molecular Biology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE