Accession Number:

ADA237256

Title:

Radiation-Induced Hemopoietic and Immune Dysfunction

Descriptive Note:

Technical rept. 16 Aug 86-31 Mar 90,

Corporate Author:

FRED HUTCHINSON CANCER RESEARCH CENTER SEATTLE WA

Personal Author(s):

Report Date:

1991-06-01

Pagination or Media Count:

34.0

Abstract:

This work was aimed at investigating radiation-induced hemopoietic and immune dysfunction in the dog model. One project was to produce monoclonal antibodies directed against canine hemopoietic precursor cells and select for pluripotent stem cells. Antibodies obtained reacted with different myeloid and erythroid precursor cells unfortunately, none of the antibodies were specific for pluripotent hemopoietic stem cells. Positive selections for these cells were performed using magnetic beads and an antibody against class II-antigen. Transplantation of class II positive marrow cells into otherwise lethally irradiated dogs led to sustained recovery in only 1 of 5 dogs. A second project established and investigated long-term marrow cultures in the dog. Culture conditions were studied and optimized, and marrow cells were transplanted into otherwise lethally irradiated dogs to investigate stem cell survival in long- term cultures. Engraftment was observed only with short-term marrow cultures.

Subject Categories:

  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE