Molecular Biology of STLV-III and HTLV-IV
Annual rept. 1 Jul 89-30 Jun 90,
DANA-FARBER CANCER INST BOSTON MA
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We have developed a novel technique for rapidly studying microheterogeneity in a population of primate immunodeficiency viruses. Proviruses are rapidly cloned by using an integration - competent cytoplasmic extract from infected cells and integration - sensitive undersize cloning vectors based on bacteriophage lambda. The details of this cloning system are presented. This method yields many clones of integrated proviral DNA, each of which represents an independent clone of provirus contained in the original infected cell population. The utility of this vector to characterize in vitro integration products of a primate immunodeficiency virus HIV-1 are illustrated as an example.
- Medicine and Medical Research