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Pathogenic and Nonpathogenic Strains of Entamoeba histolytica can be Differentiated by Monoclonal Antibodies to the Galactose-Specific Adherence Lectin
Final rept. Mar 90-Mar 1991
VIRGINIA UNIV CHARLOTTESVILLE
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Pathogenic determinants found on the outer surfaces of microorganisms determine their ability to adhere to andor invade mammalian tissues. The galactose lectin produced by Entamoeba histolytica provide the basis for development of a model system for the environmental detection of adherence and invasive properties harbored by potential threat agents. Monoclonal antibodies mAb directed against the 170 kDa heavy subunit or the amebic lectin defined six nonoverlapping epitopes. We tested 50 pathogenic and nonpathogenic strains of Entamoeba histolytica for reactivity to these mAb. By radio immunoassay mAb to epitopes 1 through 6 reacted in the radioimmunoassay with all 16 pathogenic strains tested.
APPROVED FOR PUBLIC RELEASE