Accession Number:

ADA235913

Title:

Pathogenic and Nonpathogenic Strains of Entamoeba histolytica can be Differentiated by Monoclonal Antibodies to the Galactose-Specific Adherence Lectin

Descriptive Note:

Final rept. Mar 90-Mar 1991

Corporate Author:

VIRGINIA UNIV CHARLOTTESVILLE

Personal Author(s):

• Wood-Helie, Sheila J.
• Petri, William A.

Report Date:

1991-04-01

Pagination or Media Count:

18.0

Abstract:

Pathogenic determinants found on the outer surfaces of microorganisms determine their ability to adhere to andor invade mammalian tissues. The galactose lectin produced by Entamoeba histolytica provide the basis for development of a model system for the environmental detection of adherence and invasive properties harbored by potential threat agents. Monoclonal antibodies mAb directed against the 170 kDa heavy subunit or the amebic lectin defined six nonoverlapping epitopes. We tested 50 pathogenic and nonpathogenic strains of Entamoeba histolytica for reactivity to these mAb. By radio immunoassay mAb to epitopes 1 through 6 reacted in the radioimmunoassay with all 16 pathogenic strains tested.

Descriptors:

• *TISSUES(BIOLOGY)
• DETECTION
• MAMMALS
• MODELS
• THREATS
• ADHESION
• REACTIVITIES
• SURFACES
• EXTERNAL
• MONOCLONAL ANTIBODIES
• MICROORGANISMS
• GALACTOSE
• ENTAMOEBA HISTOLYTICA
• RADIOIMMUNOASSAY
• ENVIRONMENTS

Subject Categories:

• Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE