Interaction of Leukotriene C4 and Chinese Hamster Lung Fibroblasts (V79A03 Cells). 1. Characterization of Binding
ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD
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A novel, specific, and potent biological action of leukotriene C4 LTC4 was demonstrated in the Chinese Hamster lung fibroblast cell line V79A03 V79 cells, namely the conferment of protection against subsequent irradiation. Consequently, studies were conducted to determine whether LTC4-conferred radioprotection could be attributed to a receptor-mediated phenomenon. Specific binding sites for leukotriene C4 LTC4 were identified and characterized using intact V79 cells incubated at 4 C in the presence of serine-borate, during which time conversion of LTC4 to LTD4 or LTE4 was undetectable. Binding was maximal in a broad region between pH 6.2 and 8.8 Ca2, Mg2, and Na were not required for binding, and binding was not altered by GTP, ATP, cAMP, by leukotrienes B4, D4, or E4, or by the leukotriene end point antagonists LY 171883, FPL 55712, or Revlon 5901-5.
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