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Radioprotection of Mice with Interleukin-1: Relationship to the Number of Erythroid and Granulocyte-Macrophage Colony-Forming Cells
ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD
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This report presents the results of an investigation of changes in the number of erythroid and granulocyte-macrophage colony forming cells GM-CFC that had occurred in tissues of normal B6D2F1 mice 20 h after administration of a radioprotective dose 150 ng of human recombinant interleukin-1 rIL-1. Neutrophilia in the peripheral blood and changes in the tissue distribution of GM-CFC demonstrated that cells were mobilized from the bone marrow in response to rIL-1 injection. for example, 20 h after rIL-1 injection marrow GM-CFC numbers were 80 of the numbers in bone marrow from saline-injected mice. Associated with this decrease there was a twofold increase in the number of peripheral blood and splenic GM-CFC. Also, as determined by hydroxyurea injection, there was an increase in the number of GM-CFC in S phase of the cell cycle in the spleen, but not in the bone marrow. Data in this report suggest that when compared to the spleen, stimulation of granulopoiesis after rIL-1 injection is delayed in the bone marrow.
APPROVED FOR PUBLIC RELEASE