Wound Healing by Cultured Skin Cells and Growth Factors.
Rept. for 16 May 1988-15 Nov 1989
MEMORIAL SLOAN-KETTERING CANCER CENTER NEW YORK
Pagination or Media Count:
The studies described in this report were aimed at the biochemical characterization and purification of a wound healing growth factor, named Epidermal Cell derived Factor EDF. The biological activity of EDF was found to embody enhancement of epidermal regeneration and regulation of dermal repair in skin wounds. We have accomplished the following A tissue culture bioassay that is based on the capability of fibroblasts to contract collagen gels was shown to reflect in vivo effects of EDF on dermal cells and was adopted for examination of large numbers of samples during biochemical purification steps. Supernatant fluids of epidermal cell cultures and cell extracts of epidermal cells grown in tissue culture were tested as possible sources for purification of the factor, but substituted by epidermal cells obtained from animals following a superficial wounding. Cell extracts prepared from such regenerating epidermal cells have been found to be the richest source from all tissues tested.
- Medicine and Medical Research