Accession Number:

ADA235214

Title:

Possible Mechanism for Denervation Effect on Wound Healing.

Descriptive Note:

Final rept. 1 May 1987-30 Apr 1990

Corporate Author:

INDIANA UNIV AT BLOOMINGTON

Personal Author(s):

• Mescher, Anthony L.

Report Date:

1990-12-14

Pagination or Media Count:

49.0

Abstract:

The iron-transport plasma protein transferrin is required for cell proliferation and is present in peripheral nerves. WE have tested the hypothesis that release of this factor from axons may be involved in the growth-promoting effect neurons exert on cell proliferation during certain processes of repair in poorly vascularized issues. Using regeneration in an amphibian as the model system, transferrin was purified, antibodies against it were produced, and the factor was studied qualitatively and quantitatively during nerve and limb regeneration. Immunocytochemistry showed the protein to be present in axons and Schwann cells, as well as in the perineurium of peripheral nerves. An immunoassay developed in the project revealed that the transferrin content of sciatic nerves increases dramatically during regeneration and that the protein is included in the anterograde fast transport component of axons. These results are consistent with the hypothesis that neural release of this factor may be involved in the trophic effect of nerves observed during regeneration.

Descriptors:

• *MODELS
• REPAIR
• TRANSPORT
• RELEASE
• NERVOUS SYSTEM
• ANTIBODIES
• WOUNDS AND INJURIES
• HYPOTHESES
• IMMUNOLOGY
• CELLS(BIOLOGY)
• IMMUNOASSAY
• NERVES
• HEALING
• CYTOCHEMISTRY
• EXTREMITIES
• REGENERATION(ENGINEERING)
• NERVE FIBERS
• MALNUTRITION
• SCIATIC NERVE
• PERIPHERAL NERVOUS SYSTEM
• PROTEINS

Subject Categories:

• Biology
• Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE