Development of Shuttle Vectors for Halobacteria.
Final rept. 1 Feb 1988-31 Jan 1991
DALHOUSIE UNIV HALIFAX (NOVA SCOTIA)
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We have developed systems for genetic analysis of halophilic archaebacteria. These include 1 transformation systems which we have shown to work efficiently in at least three genera of halobacteria, 2 shuttle vectors which can be selected in either halobacteria or E. coli, and 3 host systems including hundreds of mutant halobacterial strains. We have applied these methods of genetic analysis in characterizing a halobacterial HMGCoA reductase gene which is also the basis of our selection for antibiotic resistance in halobacteria and clusters of genes involved in the biosynthesis of tryptophan, histidine, arginine and leucine. These techniques have also permitted us to position dozens of genetic markers on a physical map of the Haloferax volcanii genome. Other applications include the construction of halobacterial suppressor tRNA genes by site directed mutagenesis and cloning of genes encoding halobacterial membrane proteins.
- Medicine and Medical Research