Accession Number:

ADA234910

Title:

Biological Synthesis of Substituted o-Aminophenols

Descriptive Note:

Final rept. 1 Nov 1987-31 Dec 1990

Corporate Author:

GE CORPORATE RESEARCH AND DEVELOPMENT SCHENECTADY NY BIOLOGICAL SCIENCES LAB

Personal Author(s):

Report Date:

1991-02-01

Pagination or Media Count:

82.0

Abstract:

Polybenzoxazoles are thermally stable, high modulus polymers made by the condensation of carboxylic acids with o-aminophenols. This report summarizes research to develop methods for the biosynthesis of substituted o-aminophenols from less expensive precursors such as substituted anilines, benzidine, and o- nitrophenols. One approach has been to exploit isolated enzymes to catalyze the desired reactions. Of the four enzymes we have studied for this purpose, tyrosinase shows the most promise. This enzyme has the desired catalytic activity, catalyzing the ortho hydroxylation of several anilines and benzidines, but the reaction rate is 100-fold slower than with phenols and an exogenous reductant must be added to the reaction to inhibit the enzyme-catalyzed oxidation of the desired o-aminophenol. As an alternative to this approach, we investigated the biosynthesis of o-aminophenols via bacterial reduction of o- nitrophenols. Bacterial strain PI1, a GE isolate, catalyzes the reduction of nitroaromatic compounds. This bacterium, identified as a Group D Streptococci, has broad substrate specificity for this nitro-reductase activity and good viability under high product and substrate concentrations.

Subject Categories:

  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE