Ethane Production in Copper-Deficient Rats
VETERANS ADMINISTRATION MEDICAL CENTER TUCSON AZ
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Evidence is accumulating which indicates that copper-deficient animals are prone to oxidative damage. To investigate this possibility further, we measured the production of breath ethane, a hydrocarbon by-product of lipid peroxidation, in copper deficient rats. Male, weanling Sprague-Dawley rats were fed either a purified diet which was deficient in copper CuD or the same diet made sufficient with 5 ppm of copper CuS. After 33 to 34 days the rats were placed individually in gastight metabolic cages through which ethane-free air of 100 O2 was passed. Expired ethane was absorbed onto cold, activated charcoal, liberated by heating, and measured by gas chromatography. This finding complements previous evidence that increased lipid peroxidation occurs in copper-deficient rats.