Demonstration of the Origin of Human Mast Cells from CD34+ Bone Marrow Progenitor Cells
NAVAL MEDICAL RESEARCH INST BETHESDA MD
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It has been established that murine mast cells are derived from a pluripotent bone marrow stem cell. In humans, the corresponding pluripotent cell is included in the CD34 bone marrow population. To determine whether human mast cells arise from CD34 human progenitor cells, enriched CD34 cells were cultured over agarose surfacesInterphase cultures or cocultured with mouse 3T3 fibroblasts in the presence of recombinant human rh IL-3. In the mouse, mast cell progenitor cells have been shown to originate in bone marrow and form colonies CFU-S in mouse spleen. These mast cell progenitor cells selectively adhere to mouse embryonic skin monolayers, express mRNA for the subunits of IgE receptors before phenotypic differentiation and proliferate in the presence of IL-3 and IL-4. In humans, mast cells and basophils have been cultured from bone marrow in agarose interphase cultures.
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