Interaction of Electromagnetic Fields with Biosynthetic Processes in Connective Tissue Cells
Final rept. 31 Mar 1989-30 Apr 1990
MASSACHUSETTS INST OF TECH CAMBRIDGE DEPT OF ELECTRICAL ENGINEERING AND COMPUTER SCIENCE
Pagination or Media Count:
The specific objectives of this research period were 1 determine whether chondrocytes can be maintained in long-term 2-3 months culture in agarose gel in order to test the effects of chronic as well as acute response to exposure to electromagnetic fields 2 measure chondrocyte proliferation, biosynthetic rates for proteoglycans and proteins, total accumulation of proteoglycans, and physical properties of agarosechondrocyte gel cultures 3 construct specialized chambers for application of electric fields to agarose chondrocyte disks in a standard incubator environment and 4 begin experiments to measure the effects of applied current densities over a wide range of amplitudes and frequencies on biosynthesis of proteoglycans and proteins.