Status Report on Contract N00014-89-J-1916 (Louisiana State University)
Rept. for 1 Nov 1990-28 Feb 1991
LOUISIANA STATE UNIV NEW ORLEANS SCHOOL OF MEDICINE
Pagination or Media Count:
This series of experiments demonstrated that TNF was detectable in serum up to 6 hr after a bolus injection, demonstrating rapid disappearance in the first two hr. Peripheral blood differential counts over a 48 hr period following the initial bolus injection of TNF revealed reciprocal changes in polymorphonuclear leukocytes PMN and lymphocytes in TNF-treated rats. The percent distribution of the two cell types returned to normal by 48 hr. Comparison of the time course of SO release by AMs of TNF versus saline-treated rats upon OPZ of PMA stimulation revealed no significant difference due to treatment with TNF. Since in the course of our previous investigations we were able to demonstrate a significant increase in SO release upon PMA-stimulation of AMs following TNF injection, we needed to ascertain the reason for this discrepancy. As can be observed in Figure 4, SO release upon PMA stimulation showed a high degree of variability. Analysis of the spontaneous SO release values from the Sprague-Dawley rats used in more recent studies indicated a great increase and variability in this parameter during the time period when the time course studies were completed.
- Medicine and Medical Research