Molecular Studies of Cytokine Induction.
Final rept. 1 Jun-15 Nov 89,
HENRY M JACKSON FOUNDATION ROCKVILLE MD
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While many of the molecular events in viral replication are well studied, the mechanisms by which viral infections trigger such constitutional symptoms as fever and malaise are unknown. We investigate the hypothesis that these viral constitutional symptoms can be triggered by the accumplation and release of dsRNA arising from viral replication. Total lung RNA from mice acutely infected with influenza virus but not from sham-infected mice was shown to induce fever, excess slow-wave sleep and enhanced amplitudes of electroencephalographic slow waves when injected into the rabbit brain. Influenza viral dsRNA was demonstrated in the active lung RNA preparations by reverse transcriptase-polymerase chain reaction techniques and was shown to be responsible for the rabbit responses by differential nuclease digestion.
- Medicine and Medical Research