Function of Bacterial Spore Coat Polypeptides in Structure, Resistance and Germination
Final rept. 1 Oct 1987-30 Sep 1990
PURDUE RESEARCH FOUNDATION LAFAYETTE IN
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The spore coat of Bacillus subtilis is comprised of two morphological layers each consisting of several polypeptides. One which was cloned and sequenced is processed from a precursor. A deletion of this gene resulted in some alteration in the structure of the inner coat and of the capacity to respond to a specific spore germinant. When this protein was over produced from a multicopy plasmid, the spores had a thickened inner coat and germinated poorly. The absence of another spore coat protein had extensive pleiotropic effects on the assembly of the outer spore oat. As a result, the spores were lysozyme-sensitive but they germinated at rates similar to the wild type.
- Anatomy and Physiology