Accession Number:

ADA232217

Title:

Function of Bacterial Spore Coat Polypeptides in Structure, Resistance and Germination

Descriptive Note:

Final rept. 1 Oct 1987-30 Sep 1990

Corporate Author:

PURDUE RESEARCH FOUNDATION LAFAYETTE IN

Personal Author(s):

Report Date:

1990-12-20

Pagination or Media Count:

24.0

Abstract:

The spore coat of Bacillus subtilis is comprised of two morphological layers each consisting of several polypeptides. One which was cloned and sequenced is processed from a precursor. A deletion of this gene resulted in some alteration in the structure of the inner coat and of the capacity to respond to a specific spore germinant. When this protein was over produced from a multicopy plasmid, the spores had a thickened inner coat and germinated poorly. The absence of another spore coat protein had extensive pleiotropic effects on the assembly of the outer spore oat. As a result, the spores were lysozyme-sensitive but they germinated at rates similar to the wild type.

Subject Categories:

  • Anatomy and Physiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE