A Model System for Predicting Drug Penetration thru Decayed Dentin into Inflamed Pulps
Interim rept. Oct 1986-Feb 1990
ARMY INST OF DENTAL RESEARCH WASHINGTON DC
Pagination or Media Count:
The purpose of this study was to develop an in vitro model stimulating carious dentin overlying chronically inflamed pulpal tissue for the subsequent evaluation of drug penetration into pulps in vivo. Dentin disks 1.03 thick and 10 mm in diameter, sectioned from non-carious human molars, were inserted in plastic split chambers designed as models of human teeth. To produce affected dentin, one half mL of each of four different decalcifying agents tested was placed on the occlusal side of the chamber in contact with the dentin disk and sealed. One mL of pH 7.6 phosphate buffered saline PBS was placed on the opposite pulpal side. This treatment was designed to produce moderate dentin demineralization with a gradual shift in pulpal pH from 7.6 to an acidic state of 6.8 similar to that produced in vivo by slow penetration of bacterial acids. The pH of the PBS was determined at 24 and 48 hours.
- Medicine and Medical Research