Isolation, Cloning and Expression of the Genes for Microbial Polyurethane Degradation
Technical rept. 15 Nov 1990-15 Feb 1991
TECHNICAL RESEARCH ASSOCIATES INC SALT LAKE CITY UT
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New degradation tests are in progress to determine if polyurethane Impranil-R andor urethane Sigma can be used as sole carbon source. Fifteen different cultures have been inoculated into 10 ml polyurethane- and urethane- minimal salts solutions. The DNA vector and fungal host, received from the Fungal Genetics Stock Center, Department of Microbiology, University of Kansas Medical Center are cosmid pSV50 in an E. coli host, LM83, and Neurospora crassa wild type strain 74-OR23-1VA, FGSC 2489. The genomic library will be constructed in E. coli HB101. The fungal vector host will be used for expression of the genes from HAFB-2F-Br. While the DNA isolation procedure is being optimized, concurrent experiments will be done to optimize the transformation frequency of both the E. coli competent cells and the Neurospora crassa host using previously published procedures.
- Medicine and Medical Research