Accession Number:

ADA232086

Title:

Acetylcholinesterase Inhibitors on the Spinal Cord: Actions of Organophosphates in the Mammalian Spinal Cord

Descriptive Note:

Annual rept. 1 Feb 1988-31 Jan 1989

Corporate Author:

MARYLAND UNIV BALTIMORE DEPT OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS

Personal Author(s):

Report Date:

1990-08-22

Pagination or Media Count:

29.0

Abstract:

This report describes continued studies on organophosphorus OP inhibitors of acetylcholinesterase AChE in the mammalian spinal cord. The mechanism and site of action of the potentiation andor depression of synaptic transmission by the OPs are being revealed with the use of selective agonists and antagonists of putative neurotransmitters. The spinal cords were isolated from neonatal rats 5- to 9-days old, hemisected, and placed in experimental chambers. Electrodes were attached to dorsal and ventral root pairs, and recordings of the monosynaptic reflex MSR were made from ventral roots under varying stimulation and recording paradigms to examine the actions of these agents. The ability of pralidoxime, trimedoxime or TMB-4, and diethyxime to reverse sarin-induced depression of the MSR in the isolated spinal cord from neonatal rats was examined. The reversal of sarin-induced depression was not accompanied by a regeneration of AChE activity in the spinal cord, suggesting that the reversal of sarin-induced depression of synaptic transmission in the spina cord by these oximes was unrelated to an effect on AChE or the accumulation of acetylcholine. It is more likely that the reversal resulted from blockade of cholinergic muscarinic receptors in the spinal cord.

Subject Categories:

  • Chemical, Biological and Radiological Warfare

Distribution Statement:

APPROVED FOR PUBLIC RELEASE