Workshop on Biophysics of Transmembrane Electric Fields
Rept. for 1 Jan-31 Dec , 1990
JEROME H HOLLAND LAB ROCKVILLE MD
Pagination or Media Count:
It is common accepted that efficiency of cell electrofusion is higher when cells are preliminary brought in closer contact. On this base two new approaches in cell electrofusion have been developed. The first one, fusion of cells on an electroconductive cellulose film, is very effective for spreading cells e.g. mouse L-cells that are able to attach to solid surfaces. The second, more universal, approach is electrofusion of cells in centrifugated pellets. Specific features of cell contacts and possible mechanism of electrofusion are discussed. Electric fields are supposed to initiate fusion process only in areas where plasma membranes of contacting cells are divided narrow extended gaps.
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