Hepatic Metabolism of Perfluorinated Carboxylic Acids: A Nuclear Magnetic Resonance Investigation
Annual rept. 15 Feb-14 Dec 1990
WRIGHT STATE UNIV DAYTON OH
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This research project employs nuclear magnetic resonance NMR spectroscopy to investigate the metabolic aspects of the toxicity associated perfluorinated carboxylic acids. Fluorine-19 NMR has been used to monitor the metabolic fate of perfluoro-n-octanoic acid PFOA and perfluoro-n-decanoic acid PFDA in the rat. Spectra obtained at various times following the administration of PFOA and PFDA depict the presence of the parent compounds in samples of bile, serum, urine, and liver in vivo. Urine spectra also indicate the presence of a possible metabolite which has not been identified at this time. Carbon-13 NMR is providing information regarding the effects of PFOA and PFDA on hepatic carbohydrate metabolism. Preliminary data indicate that hepatic glycogenesis is severely inhibited in rats at 3 days post treatment with PFDA. Plasma glucose and hepatic glucose appear to behave similar to control animals during the first three days post treatment with PFDA, but data obtained at days 6 and 7 indicate that hepatic glucose utilization may be slowed. These data are preliminary and experiments are currently in progress to further characterize the perfluorocarbon-induced dysfunctions of liver metabolism.