The Key Involvement of Poly (ADP-Ribosylation) in Defense Against Toxic Agents in Molecular Biology Studies
Annual technical rept. Nov 1989-Nov 1990,
GEORGETOWN UNIV WASHINGTON DC SCHOOL OF MEDICINE
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The enzyme polyADP-Rib polymerase requires DNA for activity, and it is significant that the catalytic activity of this enzyme is directly coordinated to the number of DNA strand breaks in DNA, both in vitro as well as in vivo. Significant reductions in cellular NAD levels reflect increased poly ADP-ribosylation, due to DNA breaks, and hence it is of significance to this application that 38 organophosphorus and methyl carbonate insecticides in doses as low as 0.6 parts per million cause lowering of NAD. Levels of NAD were directly correlated with teratogenesis. The poly ADP-ribosylation modification of chromatin-associated proteins plays an important function during the repair of DNA strands breaks in cells due to a variety of environmental toxic agents. This laboratory, was the first to isolate and clone a full-length cDNA for this enzyme.
- Medicine and Medical Research