Accession Number:

ADA231817

Title:

Polymyxin B(PMB)-Specific Monoclonal Antibodies

Descriptive Note:

Corporate Author:

NAVAL MEDICAL RESEARCH AND DEVELOPMENT COMMAND BETHESDA MD

Personal Author(s):

Report Date:

1991-01-23

Pagination or Media Count:

2.0

Abstract:

As has been reported earlier, PMB conjugated to either Bovine Serum Albumin BSA or Keyhole Limpet Hemocyanin KLH were very immunogenic in both rabbits and Balbc mice. The latter was not as immunogenic as the PMB-BSA conjugate largely because of it insoluble state. Both preparations have lost significant antibacterial properties suggesting that a significant number of the diaminobutyric acids were substituted by the conjugation process. The conjugated PMB preparations were still toxic to mice when given intravenously in 2 ug quantity. Multiple intraperitoneal or subcutaneous injections of 100 ug of PMB-BSA in the presence of Freunds adjuvants stimulated the production of high levels of PMB-specific antibodies in Balb-c mice.

Subject Categories:

  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE