Antibody to the RNA-Dependent DNA Polymerase of HTLV-III: Characterization and Clinical Associations
Final rept. 15 Nov 1986-14 Nov 1989
CORNELL UNIV MEDICAL COLL (WEILL) NEW YORK
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This project was designed to explore B cell epitopes of the HIV-1 polymerase which are important to the identification of in vitro immunologic responses correlating with progression of HIVdisease, and may provide targets for immune intervention. We first reported a heterogeneous immune response for inhibition of HIVreverse transcriptase activity by IgGs from HIVseropositive individuals. Loss of this inhibitor appeared to be associated with development of clinical disease, a finding which has been confirmed by at least three groups world-wide. We subsequently attempted to identify a linear sequence of the polymerase which could be used in a more rapid serologic assay to define the epitope reactivity of these anti-RT antibodies. A statistically significant correlation between anti-RT serologic reactivity and RT enzymatic inhibition was found utilizing two peptides from conserved, amino-terminus residues of the polymerase.
- Medicine and Medical Research