Determination of Dimethyl Sulfoxide (DMSO), Ethanol (ETOH), Formamide (F) and Glycerol/Formal (GF) by High Performance Liquid Chromatography (HPLC)
Technical rept. Jan-May 1988
ARMY BIOMEDICAL RESEARCH AND DEVELOPMENT LAB FORT DETRICK MD
Pagination or Media Count:
Liquid chromatographic LC methods have been developed for the determination of dimethyl sulfoxide DMSO, ethanol ETOH, formamide F, and glycerolformal GF, in aqueous samples. The samples are directly injected onto a high performance liquid chromatograph HPLC without further sample preparation. Separation of DMSO and F was achieved by using a C18 column and a mobile phase containing watermethanol. Separation of GS and ETOH was achieved by using a Dextro-Pak column and a mobile phase consisting of water. A programmable ultraviolet UV spectrophotometric detector was used to monitor the LC effluent for DMSO and F. A refractive index detector was used to monitor the LC effluent for ETOH and GF. The precision and accuracy of all methods are included in the report.
- Physical Chemistry