Xanthine Oxidase Potentiation of Reactive Oxygen Intermediates in Isolated Canine Peripheral Neutrophils
ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD
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Oxygen-derived free radicals are believed to contribute to reperfusion injury based, in part, upon results conferred by the pharmacologic administration of allopurinol. Allopurinol inhibits xanthine oxidase XO activity ischemic tissues. The possible role of XO as a pathologic mediator prompted examination of its effects on isolated peripheral canine neutrophils. In contrast to neutrophils alone, or following stimulation with phorbol myristate acetate PMA, it was determined that XO affected both the membrane potential and the metabolism significantly. Membrane potential assay showed that at 5-10 min, PMA depolarized 89-96 of the canine neutrophils between 32-48. Incubation with 0.5 Uml XO involved fewer cells 54-86, but at substantially increased cellular depolarization levels 76-90.
- Medicine and Medical Research