Endothelial Cells as a Source of Oxygen-Free Radicals. An ESR Study
ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD
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Endothelial cells were subjected to anoxiareoxygenation in order to simulate some of the free radical mechanisms occurring in ischaemiareperfusion. With ESR and spin trapping using the spin traps 5,5-dimethyl-1-pyrroline-1-oxide DMPO and 3,3,5,5-dimethyl-1-pyrroline-1-oxide M4PO, the results show that upon reoxygenation of endothelial cells, following a period of anoxia, these cells generate superoxide O2-. Cytotoxicity of the spin traps was measured by standard trypan blue exclusion methods. Cell injury or death was measured at various times during reoxygenation by lactate dehydrogenase LDH release. Experiments using oxypurinol, SOD, CAT and a combination of SOD and CAT show that while oxypurinol partially prevents spin adduct formation, the combination of SOD and CAT is more effective in doing so.
- Medicine and Medical Research