Rapid Identification of Francisella tularensis by a Fluorogenic Enzyme Immunoassay
DEFENCE RESEARCH ESTABLISHMENT SUFFIELDRALSTON (ALBERTA)
Pagination or Media Count:
A highly sensitive fluorogenic enzyme-linked immunosorbent assay FELISA, which utilizes nitrocellulose membranes as solid phase support and a fluorogenic substrate to indicate the antigen, has been adapted for the rapid identification of Francisella tularensis. Multiple samples were assayed in approximately 6 h by this method. The sensitivity achieved in a four layer sandwich assay format was 10 femtogram mL-1 of outer membrane protein and 100 colony forming units mL-1 of Francisella tularensis whole cells. The assay was highly specific for the detection of homologous and heterologous strains of Francisella tularensis while unrelated bacteria, including Brucella strains, exhibited no cross-reactivity.
- Inorganic Chemistry