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Accession Number:
ADA230819
Title:
Rapid Identification of Francisella tularensis by a Fluorogenic Enzyme Immunoassay
Descriptive Note:
Memorandum rept.
Corporate Author:
DEFENCE RESEARCH ESTABLISHMENT SUFFIELDRALSTON (ALBERTA)
Report Date:
1990-11-01
Pagination or Media Count:
36.0
Abstract:
A highly sensitive fluorogenic enzyme-linked immunosorbent assay FELISA, which utilizes nitrocellulose membranes as solid phase support and a fluorogenic substrate to indicate the antigen, has been adapted for the rapid identification of Francisella tularensis. Multiple samples were assayed in approximately 6 h by this method. The sensitivity achieved in a four layer sandwich assay format was 10 femtogram mL-1 of outer membrane protein and 100 colony forming units mL-1 of Francisella tularensis whole cells. The assay was highly specific for the detection of homologous and heterologous strains of Francisella tularensis while unrelated bacteria, including Brucella strains, exhibited no cross-reactivity.
Distribution Statement:
APPROVED FOR PUBLIC RELEASE