Accession Number:

ADA230505

Title:

Rescue of Injured Myocytes

Descriptive Note:

Annual rept. Dec 1989-Dec 1990

Corporate Author:

NORTH CAROLINA UNIV AT CHAPEL HILL DEPT OF CELL BIOLOGY AND ANATOMY

Personal Author(s):

Report Date:

1990-11-30

Pagination or Media Count:

10.0

Abstract:

The crucial events leading to loss of cell viability after hypoxic and toxic injury remain poorly understood. Cell death is rarely synchronous, and rapidly occurring events leading to irreversible injury may be obscured when large cell populations are studied. To overcome this drawback, single cultured hepatocytes and myocytes during hypoxic and toxic stress were studied using a new, rapidly developing technology called Multiparameter Digitized Video Microscopy MFBM. In MDVM, living cells are labeled with multiple fluorescent probes. Each probe responds to a defined cellular parameter by emitting fluorescence of a specific wavelength. 2-dimensional images of the fluorescence of each probe are digitized and stored during the course of cell injury. In this way, several cellular parameters are monitored quantitatively over time.

Subject Categories:

  • Toxicology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE