Accession Number:

ADA230411

Title:

Genetic Engineering of Clostridium Difficile Toxin a Vaccine

Descriptive Note:

Annual rept. 15 Jun 1989-14 Jun 1990

Corporate Author:

SPELMAN COLL ATLANTA GA

Personal Author(s):

• Muldrow, Lycurgus L.
• Johnson, Joe

Report Date:

1990-08-16

Pagination or Media Count:

16.0

Abstract:

The long range goal of this project is to use C. difficile toxin A as a model to add technical information in the area of vaccine biotechnology using recombinant DNA techniques. This will be accomplished in part by predicting epitopes from the DNA sequence of toxin A, synthesizing DNA for these predicted regions, and testing their antigenic activity in expression vectors. Part of the toxin A gene has been cloned and sequenced. A total of twelve different epitodes have been predicted, synthesized, cloned into lambda gtll and tested for antigenicity. Alternative procedures for epitode mapping has been described and initiated.

Descriptors:

• *CLOSTRIDIUM
• *GENETIC ENGINEERING
• *VACCINES
• SYNTHESIS
• LONG RANGE(TIME)
• DEOXYRIBONUCLEIC ACIDS
• SEQUENCES
• LONG RANGE(DISTANCE)
• CLONES
• BIOTECHNOLOGY
• ANTIGENS
• TOXINS AND ANTITOXINS

Subject Categories:

• Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE