Characterization of Ground Squirrel Retinal Ganglion Cells
Progress rept. 1 Jul-31 Dec 1990
PUERTO RICO UNIV SAN JUAN INST OF NEUROBIOLOGY
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Our work during this period mainly involved setting up new electrophysiological equipment for intracellular injections of retinal ganglion cells and performing immunohistochemical studies on the ground squirrel retina. We completed experiments relating to the nature of neurotransmittersmodulators in this retina, with particular interest in the ganglion cell layer. We investigated 1 the distribution of GABAergic neurons using antibodies against GABA and GAD and 2 the distribution of catecholamines using an antibody against tyrosine hydroxylase TH. Retinal sections and wholemounts were incubated with the particular antibody and then processed using immunofluorescence andor the avidin-biotin method.
- Medicine and Medical Research