Diagnosis and Prevention of Infection by Nairoviruses
Final rept. 17 Sep 1987-16 Sep 1990
NATURAL ENVIRONMENT RESEARCH COUNCIL SWINDON (UNITED KINGDOM)
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The aims of the contract were to elucidate the coding and replication strategies of nairoviruses, and determine whether reagents derived from Dugbe DUG virus can be used to detect Crimean Congo hemorrhagic fever CCHF viral infections. The small S segment of DUG virus was shown to comprise 1712 nucleotides and encode a single protein, the 49.9K nucleoprotein N, in viral complementary vc RNA. No sequence similarity was detected with representatives of the Hanta-, Bunya-, or Phlebovirus genera of the Bunyaviridae family. Approximately 75 i.e. 4953 nucleotides of the sequence of the M segment of DUG virus was derived and revealed a single open reading frame ORF in the vc RNA. The gene encoding the G1 structural glycoprotein appears to be located at the carboxy-terminal end of the M segment ORF. Cloned cDNA derived from the S and M genomic RNA segments of DUG virus was used to prepare 32p-labelled RNA probes.
- Medicine and Medical Research