Cutaneous Toxicity of Mustard and Lewisite on the Isolated Perfused Porcine Skin Flap
Rept. for 30 Sep 1987-1 Apr 1990,
NORTH CAROLINA STATE UNIV AT RALEIGH SCHOOL OF VETERINARY MEDICINE
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This report describes the utilization of the isolated perfused porcine skip flap IPPSF to characterize the cutaneous toxicity of sulfur mustard XHD and lewisite XL. The IPPSF is an humane, alternative, in vitro, animal model consisting of an isolated perfused skin preparation with intact and viable epidermis and dermis in association with a functional microcirculation. Task 1 of the present contract was to characterize the dose-response profile of both vesicants in 53 IPPSFs using biochemical, physiological, and morphological light and transmission electron microscopy endpoints. Twenty-four IPPSFs were treated with 200 microliters of either 5.0, 2.5, 1.25, 0.2, 0.14, or 0.07 mgml XL, and 29 IPPSFs were treated with 200 micron l of either 10.0, 5.0, 2.5, 1.25, 0.5 or 0.2 mgml XHD. At least four replicates were performed per compound, and four untreated IPPSFs served as controls. Experiments lasted 8 hours. Both XL and XHD resulted in a reduction in the cumulative glucose utilization and an increased vascular resistance as compared to controls. The high-dose groups for both agents consistently showed a marked alteration in both parameters.
- Medicine and Medical Research