Accession Number:

ADA229761

Title:

Characterization of the Voltammetric Response at Intracellular Carbon Ring Electrodes

Descriptive Note:

Technical rept.

Corporate Author:

PENNSYLVANIA STATE UNIV UNIVERSITY PARK DEPT OF CHEMISTRY

Report Date:

1990-11-05

Pagination or Media Count:

30.0

Abstract:

The Intracellular response of ultrasmall carbon ring electrodes has been investigated. Adsorption of high molecular weight species e.g. proteins, lipids, sugars, etc. on the electrode surface appears to result in deterioration of the amperometric signal, making in vivo voltammetric measurements difficult. Assuming a linear dependence between the degree of electrode fouling and the number of scans taken in the neuronal microenvironment an analytical method based on both pre- and post-calibration data is presented to evaluate the response obtained at deteriorating ultrasmall carbon ring electrodes during intracellular voltammetry. Additionally, polyester sulfonic acid-coated carbon ring electrodes have been used in an attempt to increase selectivity in intracellular voltammetry, and to reduce the effects of electrode fouling. In vitro results results obtained in solutions of dopamine, dihy- droxyphenylacetic acid and asorbic acid indicate some exclusion of anionic species from the electrode by the negative charged polymer film. Moreover, use of polymer-coated electrodes in vivo results in a decrease in the percentage of electrode fouling to where the degredation of the response is only 45. This leads to a more reproducible response and better detection limits compared to that of naked electrodes. Keywords In vivo analysis, Voltammetric measurements, Ultrasmall carbon ring electrode.

Subject Categories:

  • Biochemistry
  • Genetic Engineering and Molecular Biology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE