Accession Number:

ADA229651

Title:

Structure and Expression of Genes for Flavivirus Immunogens

Descriptive Note:

Annual summary rept. 14 Jun 1987-14 Jun 1988,

Corporate Author:

MASSACHUSETTS UNIV AMHERST DEPT OF BIOCHEMISTRY

Report Date:

1990-06-28

Pagination or Media Count:

10.0

Abstract:

Progress during the report period included 1 Extension of the nucleotide sequence analysis of the Japanese encephalitis JE and dengue serotype 1 DEN-1 genetic material 2 expressed in E. coli both fusion and non-fusion derivatives of the envelope structural glycoprotein E and the non- fusion derivatives of the envelope structural glycoprotein E and the nonstructural glycoprotein NS1 3 used deletion analysis to define the minimal amino acid sequences necessary for the binding of anti-JE and anti-DEN-1 monoclonal antibodies 4 determined that fusion derivatives of JE E and NS1 proteins expressed in E. coli do not provide protective immunity in mice 5 developed nonradioactive nucleic hybridization probes for sensitive detection of DEN RNAs in virus-infected cells 6 initiated collaborative efforts to express JE and DEN-1 antigens in baculovirus and vaccinia virus expressions systems 7 established a culture facility for the growth and analysis of DEN-1. JS

Subject Categories:

  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE