Trimester Report on Contract Number N00014-89-J-3073 (Massachusetts General Hospital)
Rept. for 1 Jul-7 Nov 1990
MASSACHUSETTS GENERAL HOSPITAL BOSTON
Pagination or Media Count:
Work Summary A. Purification of LPS binding factors in tolerant serum by affinity chromatography B. Purification of the LPS binding factor by ultracentifugation and chromatography using our radioimmunoassay C. Neutralization of LPS by tolerant serum as assessed by tumor necrosis factor production -- In continuation of our studies involving LPS and the macrophage membrane, we have set up the assay as described in the original grant and measured the production of TNF by a murine macrophage cell line. LPS was incubated in each serum and then exposed to RAW264.7 cells for four hours, at which time supernatants were harvested and assayed for TNF.
- Medicine and Medical Research