Accession Number:

ADA229002

Title:

Use of Liposomes for Directed Drug Delivery Against Entamoeba Histolytica

Descriptive Note:

Midterm rept. 1 Jul 1988-1 Jan 1990

Corporate Author:

MOREHOUSE SCHOOL OF MEDICINE ATLANTA GA

Personal Author(s):

Report Date:

1990-01-20

Pagination or Media Count:

20.0

Abstract:

The ability of purified glycosphingolipids to enhance liposome stimulated Entamoeba histolytica actin polymerization was assessed as a means to define the specificity of mammalian cell membrane lipid glycan recognition by this parasite. Synthetic liposomes containing a variety of individual glycosphingolipids bearing neutral, straight chain oligomeric glycans with galactose or N-acetylgalactosamine termini stimulated rapid 90 sec polymerization of amoeba actin. Glycans with terminal N-acetylgalactosamine residues were not, or only weakly, stimulatory. Glycans with glucose, N- acetylgalactosamine, galatose and N-acetylgalactosamine as the penultimate residue were recognized. Attachment of N-acetylneuraminate to the terminal residue of stimulatory glycosphingolipid eliminated activity attachment of fucose to the penultimate sugar reduced activity. Subject terms Liposomes Entamoeba histolytica Drugs Glycosphingolipids Phagocytosis Lipids Actin.

Subject Categories:

  • Biochemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE