Use of Liposomes for Directed Drug Delivery Against Entamoeba Histolytica
Midterm rept. 1 Jul 1988-1 Jan 1990
MOREHOUSE SCHOOL OF MEDICINE ATLANTA GA
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The ability of purified glycosphingolipids to enhance liposome stimulated Entamoeba histolytica actin polymerization was assessed as a means to define the specificity of mammalian cell membrane lipid glycan recognition by this parasite. Synthetic liposomes containing a variety of individual glycosphingolipids bearing neutral, straight chain oligomeric glycans with galactose or N-acetylgalactosamine termini stimulated rapid 90 sec polymerization of amoeba actin. Glycans with terminal N-acetylgalactosamine residues were not, or only weakly, stimulatory. Glycans with glucose, N- acetylgalactosamine, galatose and N-acetylgalactosamine as the penultimate residue were recognized. Attachment of N-acetylneuraminate to the terminal residue of stimulatory glycosphingolipid eliminated activity attachment of fucose to the penultimate sugar reduced activity. Subject terms Liposomes Entamoeba histolytica Drugs Glycosphingolipids Phagocytosis Lipids Actin.