Sulfur Mustard (SM) Lesions in Organ-Cultured Human Skin: Markers of Injury and Inflammatory Mediators
Final rept. 17 Feb 1987-16 Apr 1990
JOHNS HOPKINS UNIV BALTIMORE MD SCHOOLOF HYGIENE AND PUBLIC HEALTH
Pagination or Media Count:
The paranuclear vacuolization test for injury to human skin. Full thickness specimen of human skin 1.0 cm 2 were topically exposed to 10 yl of 0.03 to 1.0 SM and organ-cultured for 24 hr at 36 C. There was a straight-line dose-response relationship between the above concentrations of SM and the number of paranuclear vacuoles seen historically in the epidermis. Within the same SM dosage range, there was also a proportional decrease in 14 C-leucine incorporation by the explants. Early mediators of inflammation produced by SM in human skin. Culture fluids from SM-exposed and control full-thickness human skin explants contained similar amounts of angio-tensin-converting enzyme, tryspin- like and chymotryspin-like proteases, acid phosphatase, B-glucuronidase, B- galactosidase, lysozyme, deoxyribonuclease, ribonuclease, interleukin 1 and lactic dehydrogenase. However, the culture fluids from the SM-exposed explants contained increased amounts of histamine, plasminogen activator PA, and usually, prostaglandin E2 PGE2, compared to culture fluids from control explants. Keywords Sulfur mustard, full-thickness human skin explants in organ culture histology histochemistry Inflammatory medidators Viability of human skin at 4 C.
- Medicine and Medical Research