Accession Number:

ADA224981

Title:

Acetylcholinesterase Chirality and Cellular Mechanisms of Organophosphonate Toxicity

Descriptive Note:

Final rept. 1 Jun 1985-31 Mar 1990

Corporate Author:

STATE UNIV OF NEW YORK AT BUFFALO DEPT OF BIOCHEMICAL PHARMACOLOGY

Personal Author(s):

Report Date:

1990-06-11

Pagination or Media Count:

36.0

Abstract:

These studies concern the chiral reactivity of AchE, and examine the topography of the enzyme subunit. Several independent kinetic and equilibrium indices of ligand orientation are employed bimolecular inhibition, oxime reactivation, aging, and equilibrium dissociation constants. Organic synthesis is an integral part of these studies and we developed several families of achiral and chiral, and fluorescent and non-fluorescent organophosphonates and reversible noncovalent fluorescent probes. These agents are employed in study of AchE from Torpedo, as one means for probing subunit topography, as well as in in vivo and in vitro studies concerning cellular regulation of AchE. Keywords Acetylcholinesterase Organophosphates Organophosphonates Chirality Cellular toxicity.

Subject Categories:

  • Toxicology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE