Cellular and Molecular Mechanisms of High Pressure Inotropy in Cardiac Muscle
Annual rept. 1 Aug 1989-31 Jul 1990
STATE UNIV OF NEW YORK AT BUFFALO DEPT OF PHYSIOLOGY
Pagination or Media Count:
We have spent the past year designing, building and testing the components of a microfluorimeter system for measuring cytosolic calcium in single cardiac cells at high hydrostatic pressure. The central element of the system is a 75 microliter cell chamber fitted with windows and placed in the optical axis of a Zeiss inverted microscope. An optical network has been devised to record simultaneously the fluorescent emission from FURA-II, a calcium reporter dye, and sarcomere spacing from differential interference contrast imaging. To achieve the required microsecond time resolution and sub-micrometer space resolution we have integrated optical, mechanical and electrical components using an 8038680387 central processor with custom interface hardware and software. Details of these technical developments are provided in our annual report. Keywords Membranes, Cardiac muscle, Contraction force, Calcium, Inotropy, Myocyte, Pressure.
- Medicine and Medical Research