Effects of Pressure on Membrane-Associated Receptors and Effector Elements
Annual rept. Jul 1989-Jun 1990
OREGON STATE UNIV CORVALLIS COLL OF PHARMACY
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To assess the effects of hydrostatic pressure on transmembrane signal transduction, we hace examined the A1 adenosine receptor - G protein - adenylyl cyclase complex in brain tissue of marine teleost fishes which occur at different depths. We have used two congeneric species, Sebastolobus alascanus and S. altivelis and the deeper-living Antimora rostrata. The function of adenylyl cyclase and modulation of basal adenylyl cyclase activity by A1 adenosine receptor agonists of ATP for adenylyl cyclase is pressure of 272 atm in brain membranes from A. rostrata. The K sub m of ATP for adenylyl cyclase is pressure sensitive in te Sebastolobus species. However, the adenylyl cyclase of the deeper-living S. altivelis is less affected by pressure increases. Pertussis toxin-catalyzed 32P ADP-ribosylation of G proteins occupancy of the muscarinic cholinergic and adenosine receptors. The phospholipid and fatty acid compositions of the Sebastolobus species are similar.